We have seen errors in some of the PbHiT tagging vector sequences generated by this script. This error occurs only when the top ranked gRNA (displayed first) in on the reverse strand. For PbHiT tagging vectors we kindly ask you to double check the sequences manually. We are currently working on improving this script
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This site provides a database of knockout designs targeting the entire Plasmodium berghei protein encoding genome using the PbHiT CRISPR system as described in Jonsdottir et al. DOI: https://doi.org/10.1101/2024.04.20.590404 Synthetic fragments are ranked by guide RNA scoring.
Generated by the Bushell lab at Umeå University. http://www.bushelllab.com/