We have discovered an error in the PbHiT designs generated from 22 January 2025 until 22 April 2025 for knockout designs and 11 June 2025 for tagging designs. This primarily affects constructs where the gRNA is on the reverse strand where the tool makes a mistake in the design of the homology arms that renders the construct faulty. Please check all construct designs downloaded during this time period carefully. We encourage all users to routinely check all designs by alignment against target genomic sequences.